Bento 4 manual

Overall, you need four different primers that flank each side of the DNA you are trying to copy. The exact temperature depends on the experiment, and is known as the annealing temperature. At this point, the primers 2 will attach, or anneal , to their binding positions on the single strands of the template DNA. In the next step, the reaction moves to an optimal temperature for the polymerase enzyme 1 , from which PCR takes its name, to start working.

This process is repeated about times. Each cycle doubles the copies of the DNA targeted by the primers. This allows for the design of identification experiments. If some primers do not work, it is possible that the DNA sample did not contain the right DNA for the targeted sequence.

Bento Lab has a thermocycler module on the right side. This is a programmable heat block, which fits 0. This size tube is the standard tube to mix the ingredients for PCR. By default, Bento Lab has two standard protocols: One for PCR, and a simpler heat block program, which is useful for incubation and heating samples.

For example, the latter is used in the DNA Extraction method of this kit. Here you can edit and change all the parameters of the protocol 1 , give the custom protocol a name and save it 2 , and start the thermocycler program 3.

From the graphical representation, you can see the three stages that are repeated 30 times. You can find out more about how to edit a protocol, and use the interface in this detailed chapter of the Bento Lab manual , which has animations demonstrating changing temperatures and durations, cycles, etc. During PCR, the heated lid is heated to a higher temperature that the heat block where the tubes are placed, so the top of the tube is hotter than the bottom.

If there was no heated lid, the reaction mix inside the tube would soon evaporate and condensate inside the tube. For all experiments using PCR in the Biotechnology Kit, you will be provided with a specific set of primers. This bead contains:. Finally, you will prepare, as part of each experiment, specific template DNA 3.

These need to be mixed in precise proportions, for which you will need the micropipette 4. We use cookies to improve your browsing experience on our website. You can find more information in our Privacy Policy here, and in our Cookies Policy here. You can view your cookies settings here. Bento Lab is a portable mobile genomics setup. The compact laboratory is equipped with thermal cycler 1 a microcentrifuge 2 , a blue LED transilluminator 3 and a gel electrophoresis power supply used with an external gel electrophoresis container 4.

The system is operated using an intuitive on-board graphical interface 5 , and comes with a convenient tube rack 6.

Bento Lab is a professional laboratory device, and although it is also used in educational settings, it is not a toy. Read this manual carefully, in particular sections on safety, before using the device. This chapter of the Bento Lab user manual describes the main physical characteristics of Bento Lab, and notes on installation.

Overview of the Bento Lab User Interface. Explains the function of each button, and common elements on the graphical user interface used to operate the device. User manual chapter for the Bento Lab centrifuge module, including a physical specification, operation guide, information on safety and balancing. User manual chapter for the Bento Lab gel electrophoresis and transilluminator module.

Includes a physical specification, information on casting gels and connecting the gel box, and operation guide for the interface. Explanation of safety symbols, notes on maintenance, technical specifications and warnings.

This collection contains useful documents to download that can be helpful for owners of Bento Lab. Advanced Options for Bento Lab. Connecting to Wifi, Installing software updates, Replaying the user tutorial, Checking serial number and configuration details, Restoring factory settings.

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